Metabolism of 6:2 Fluorotelomer Alcohol by CYP 2A6

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  • Oxidation is one of the major mechanisms leading to the bioactivation of toxicants [1]. Identification of the CYPs driving the oxidation could be used to identify the possibility of localized toxicity. This project identifies CYP 2A6 as a possible enzyme for the oxidation of 6:2 FTOH. The Michaelis Menten curve generated a KM and VMax of 4076.4 ± 581.9 ng/mL and 68.8 ± 2.8 ng/mL/min respectively. Once inhibited with tranylcypromine, HCl, the KM and VMax were determined to be 8796.2 ± 1366.1 ng/mL and 69.5 ± 4.1 ng/mL/min, representing competitive inhibition. We demonstrated that CYP 2A6 was responsible for 6:2 FTOH metabolism using human recombinant assays with purified CYP 2A6. These assays yielded a 6:2 FTOH metabolic conversion rate of 0.42 ng/mL/min. This rate significantly decreased with the addition of Tranylcypromine HCl. This confirms CYP 2A6 as an active enzyme for the metabolism of 6:2 FTOH in the human liver.

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  • Copyright © 2021 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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  • 2021


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