Characterization and Application of a Histamine Aptamer-Based BiosensorPublic Deposited
Downloadable ContentDownload PDF
- Resource Type
Histamine is a biogenic amine, identified as a natural contaminant in food and alcoholic beverages, with high levels eliciting allergy-related conditions. Exposure to allergens can lead to abnormal high levels of histamine in the body that is detectable in blood and urine. A rapid histamine biosensor that can detect histamine levels in food, beverages and also be used as a diagnostic tool for allergy-related conditions will be an ideal point of care testing tool. In addition, histamine has been identified as a close-range aggregation pheromone component in bed bug feces. A portable biosensor that detects bed bugs in these settings is in demand. Nano-aptamer based biosensors have previously been used to detect wide range of targets. In this study, histamine-binding aptamers were characterized. A head-to-head comparison of binding affinity and specificity for these group of aptamers were conducted, and the aptamer candidates best suited for biosensor application was selected.
- Thesis Degree Level
- Thesis Degree Name
- Thesis Degree Discipline
- Rights Notes
- Copyright © 2021 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
- Date Created
- In Collection: