Development and Characterization of DNA Origami Nanostructures and their Application as an Aptamer-Mediated Targeted Delivery System

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  • The predictable geometries and intermolecular interactions of DNA have led to the development of many arbitrary nanoscale objects, including a class termed DNA origami. DNA origami nanostructure fabrication occurs through the folding of long, single-stranded DNA using short, single stranded oligonucleotides. Detailed herein is the development and characterization of DNA origami nanostructures with site-specific modifications for aptamer-mediated binding of target molecules. Molecular self-assembly is performed by thermal annealing and fabricated objects are purified by centrifugal filtration through molecular weight cut-off membranes. Nanostructures are characterized by agarose gel electrophoresis, UV-Vis quantification, and visualization by atomic force microscopy. Thrombin-binding aptamers, TBA15 and TBA29, were captured to the surface of rectangular DNA origami nanostructures and were incubated with the target, thrombin, to demonstrate the site-specific binding of target molecules. DNA origami nanostructures functionalized with aptamers for specific target recognition bear attractive potential for application in targeted therapies and targeted delivery of molecular payloads.

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  • Copyright © 2022 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
Date Created
  • 2022


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