Examining the role of the promoter region of het-6 in escape from heterokaryon incompatibility in Neurospora crassa

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  • Heterokaryon incompatibility (HI) is a form of nonself recognition during the formation of heterokaryons – cells containing genetically dissimilar nuclei. HI is controlled by het loci; of these, het-6 is a supergene complex encoding two tightly-linked incompatibility genes, un-24 and het-6, with allelic variants Oak Ridge (OR) and Panama (PA). het-6 encodes a HET domain protein with no known function outside of incompatibility, whereas un-24 encodes the large subunit of ribonucleotide reductase. Strains incompatible at het-6 can ‘escape’ from HI by mutation in the het-6 gene or suppress HI by mutation in vib-1, a transcription factor. This study examined the possible role of the het-6 promoter region in initiating escape, by introducing an ectopic het-6OR allele into a strain with a null endogenous het-6OR allele. Self-incompatible transformants exhibited delayed escape. A vib-1 mutant allele partially suppressed incompatibility at the ectopic het-6OR. This study provides insight into the process of escape.

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  • Copyright © 2016 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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  • 2016


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