Inhibitory Effects of Hydrolyzed Oat Bran Proteins on Human LDL Oxidation and Their Bile Acid Binding Capacity

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  • The aim of this project was; 1) to produce and characterize various hydrolyzed proteins from oat brans; 2) determine their antioxidant, metal binding and bile biding capacities as well their ability to inhibit low-density lipoprotein (LDL) oxidation. Medium oat bran samples treated with (cellulase and viscozyme) were digested with five proteases protamex, alcalase, flavourzyme, pepsin and pepsin+pancreatin. VPI-pepsin better scavenged ROO• radicals (496.77±5.83 μM(TE)/g) while VPI-flavourzyme and VPI-pepsin had better quenching for HO• (27.95 ±1.580 and and O2•- (45.31±6.6%) radicals, respectively. VPI-protamex exhibited the best Cu2+ chelating capacity (59.83±1.40%). All hydrolysates protected human LDL against Cu2+ mediated oxidation by reducing the concentration of hydroperoxides from 158.4 to 74.4-97.7 μMH2O2/mg. There was a binding of up to 46.3% for taurodeoxycholate and taurocholate. VPI alcalase proteins displayed the highest activity in the most assay and consequently separated into eleven fractions (F1-F11) (HPLC).

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  • Copyright © 2019 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
Date Created
  • 2019


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