Electrophysiological Profile of Differentiating Human Central Canal Ependymal Stem-Progenitor Cells

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  • Stem cell transplant and niche manipulation therapies are a promising tool for repairing damage from SCI and other neurodegenerative conditions. Since immunocytochemistry alone cannot adequately determine whether stem cells have differentiated into mature neurons, we used patch-clamp electrophysiology to assess passive and active electrical properties of spinal cord central canal stem cells. Human epSPCs differentiate towards a majority neuronal fate, with no action potential firing from two to ten weeks in vitro regardless of differentiation media. Passive membrane properties failed to reach typical mature neuron levels. The majority of cells showed voltage-dependent spontaneous synaptic currents with reversal near 0 mV, outward rectification, and decay kinetics consistent with excitatory glutamatergic responses. In future, research will examine the required timeline and most effective differentiation media for development of active membrane properties. Identification of receptor subtypes responsible for observed synaptic currents is required. This will inform future stem cell-based treatments of neurological disorders.

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  • Copyright © 2018 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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  • 2018


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