Application of amplified fragment length polymorphism-derived genetic markers to address life-history hypothesesPublic Deposited
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The focus of this thesis was to optimize Amplified Fragment Length Polymorphism (AFLP) technologies and develop AFLP-based genetic markers to address specific life-history questions related to the damselfly, Nesobasis rufostigma. Optimization of AFLP marker production targeted small (< 0.25 mm3) tissue samples and focussed on several key steps in the process. In order to reduce the number of analyzable markers to a more manageable number, a process was developed and evaluated by which discrete PCR-based markers were obtained from AFLPs. The genetic markers developed in this study were used to assess life-history questions such as relatedness, paternity and mode of reproduction with the damselfly N. rufostigma. Through genotypic comparisons of female adults and their offspring it was established that N. rufostigma reproduces sexually, and not via parthenogenesis, as originally hypothesized. These comparisons also suggested that some broods were sired by multiple males.
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