Application of amplified fragment length polymorphism-derived genetic markers to address life-history hypotheses

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  • The focus of this thesis was to optimize Amplified Fragment Length Polymorphism (AFLP) technologies and develop AFLP-based genetic markers to address specific life-history questions related to the damselfly, Nesobasis rufostigma. Optimization of AFLP marker production targeted small (< 0.25 mm3) tissue samples and focussed on several key steps in the process. In order to reduce the number of analyzable markers to a more manageable number, a process was developed and evaluated by which discrete PCR-based markers were obtained from AFLPs. The genetic markers developed in this study were used to assess life-history questions such as relatedness, paternity and mode of reproduction with the damselfly N. rufostigma. Through genotypic comparisons of female adults and their offspring it was established that N. rufostigma reproduces sexually, and not via parthenogenesis, as originally hypothesized. These comparisons also suggested that some broods were sired by multiple males.

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  • Copyright ¬© 2010 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
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  • 2010

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