Mass Spectrometry-based Anaylsis to Investigate the Pharmacokinetics and Proteomic Properties of a Viral Sensitizer

Public Deposited
Resource Type
  • Attenuated oncolytic viruses (OVs) are a promising alternative cancer therapy to mainstream methods such as radiotherapy and chemotherapy. OV therapy takes advantage of the defective antiviral response present in most cancer cells however heterogeneity amongst target cells and attenuation of OVs to increase their safety profiles has limited the efficacy of this treatment. We have developed novel small molecules named viral sensitizers (VSes) capable of enhancing viral infection and cancer-specific cell death. Liquid chromatography-mass spectrometry (LC-MS) methods were developed to study the pharmacokinetics (PK) and metabolomic activity of VSe1-28 via time course experiments. Parallel to this work, proteomic experiments were conducted to confirm the molecular target and mechanism of action of VSe1-28 through MRM and Auto-MS/MS methods. These new findings will aid in improving VSes and progress preclinical studies for clinical use in combination with OVs.

Thesis Degree Level
Thesis Degree Name
Thesis Degree Discipline
Rights Notes
  • Copyright © 2021 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.
Date Created
  • 2021


In Collection: